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human starr seq screening vector  (Addgene inc)


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    Addgene inc human starr seq screening vector
    Human Starr Seq Screening Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+starr+seq+screening+vector/hSTARR-seq_SCP1+vector_blocking+4+(Plasmid+%2399319)/pm35594906-509-3-11
    Average 90 stars, based on 2 article reviews
    human starr seq screening vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Compatibility logic of human enhancer and promoter sequences
    Article Snippet: We purified the PCR products using 0.8X volume of AMPure XP beads (Beckman Coulter, A63881) and pooled the reactions together while keeping enhancers and promoters separate. .. We digested the human STARR-seq screening vector (hSTARR-seq_SCP1 vector_blocking 4, Addgene #99319) with both Thermo SgrDI and BshTI (AgeI) (replaced with enhancer sequence), then NEB KpnI and ApaI (replaced with promoter sequence), with purification using 0.8X volume AMPure XP after each digestion. .. We then recombined 500ng of this digestion (including ∼4.4kb of backbone vector and 250bp of filler sequence including a spliced region and truncated GFP ORF) with 150ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 hour at 50°C in a 40uL reaction and purified the reaction using 1X volume AMPure XP with 3 total ethanol washes.

    Article Title: Compatibility rules of human enhancer and promoter sequences.
    Article Snippet: We purified the PCR products using 0.8× volume of AMPure XP beads (Beckman Coulter, A63881) and pooled the reactions together while keeping enhancers and promoters separate. .. We digested the human STARR-seq screening vector (hSTARR-seq_ SCP1 vector_blocking 4, Addgene, 99319) with both Thermo SgrDI and BshTI (AgeI) (replaced with enhancer sequence), then NEB KpnI and ApaI (replaced with promoter sequence), with purification using 0.8× volume AMPure XP after each digestion. .. We then recombined 500 ng of this digestion (including about 4.4 kb of backbone vector and 250 bp of filler sequence, including a spliced region and truncated GFP open reading frame) with 150 ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 h at 50 °C in a 40 μl reaction.

    Sequencing:

    Article Title: Compatibility logic of human enhancer and promoter sequences
    Article Snippet: We purified the PCR products using 0.8X volume of AMPure XP beads (Beckman Coulter, A63881) and pooled the reactions together while keeping enhancers and promoters separate. .. We digested the human STARR-seq screening vector (hSTARR-seq_SCP1 vector_blocking 4, Addgene #99319) with both Thermo SgrDI and BshTI (AgeI) (replaced with enhancer sequence), then NEB KpnI and ApaI (replaced with promoter sequence), with purification using 0.8X volume AMPure XP after each digestion. .. We then recombined 500ng of this digestion (including ∼4.4kb of backbone vector and 250bp of filler sequence including a spliced region and truncated GFP ORF) with 150ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 hour at 50°C in a 40uL reaction and purified the reaction using 1X volume AMPure XP with 3 total ethanol washes.

    Article Title: Compatibility rules of human enhancer and promoter sequences.
    Article Snippet: We purified the PCR products using 0.8× volume of AMPure XP beads (Beckman Coulter, A63881) and pooled the reactions together while keeping enhancers and promoters separate. .. We digested the human STARR-seq screening vector (hSTARR-seq_ SCP1 vector_blocking 4, Addgene, 99319) with both Thermo SgrDI and BshTI (AgeI) (replaced with enhancer sequence), then NEB KpnI and ApaI (replaced with promoter sequence), with purification using 0.8× volume AMPure XP after each digestion. .. We then recombined 500 ng of this digestion (including about 4.4 kb of backbone vector and 250 bp of filler sequence, including a spliced region and truncated GFP open reading frame) with 150 ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 h at 50 °C in a 40 μl reaction.

    Purification:

    Article Title: Compatibility logic of human enhancer and promoter sequences
    Article Snippet: We purified the PCR products using 0.8X volume of AMPure XP beads (Beckman Coulter, A63881) and pooled the reactions together while keeping enhancers and promoters separate. .. We digested the human STARR-seq screening vector (hSTARR-seq_SCP1 vector_blocking 4, Addgene #99319) with both Thermo SgrDI and BshTI (AgeI) (replaced with enhancer sequence), then NEB KpnI and ApaI (replaced with promoter sequence), with purification using 0.8X volume AMPure XP after each digestion. .. We then recombined 500ng of this digestion (including ∼4.4kb of backbone vector and 250bp of filler sequence including a spliced region and truncated GFP ORF) with 150ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 hour at 50°C in a 40uL reaction and purified the reaction using 1X volume AMPure XP with 3 total ethanol washes.

    Article Title: Compatibility rules of human enhancer and promoter sequences.
    Article Snippet: We purified the PCR products using 0.8× volume of AMPure XP beads (Beckman Coulter, A63881) and pooled the reactions together while keeping enhancers and promoters separate. .. We digested the human STARR-seq screening vector (hSTARR-seq_ SCP1 vector_blocking 4, Addgene, 99319) with both Thermo SgrDI and BshTI (AgeI) (replaced with enhancer sequence), then NEB KpnI and ApaI (replaced with promoter sequence), with purification using 0.8× volume AMPure XP after each digestion. .. We then recombined 500 ng of this digestion (including about 4.4 kb of backbone vector and 250 bp of filler sequence, including a spliced region and truncated GFP open reading frame) with 150 ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 h at 50 °C in a 40 μl reaction.



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